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Application: / Monitor IFNγ and INFα-induced activity./ Screen molecules that block the IFN signaling pathway./ Investigate IL-12 related signaling pathway after introducing STAT4 and IL-12 receptor complex in this cell line.
Background: IRF1 (interferon regulatory factor 1), also known as MAR, is a transcription factor ubiquitously expressed at low levels, which is responsive to IFN (interferon). It regulates pro-inflammatory responses, cell development and differentiation. Depending on the context, it can act as a tumor suppressor or oncogene, and has been linked also to auto-immune, inflammatory and metabolic disorders. It has been shown that IL-12 induces the binding of STAT4 (Signal transducer and activator of transcription 4) to the GAS (gamma-activated sites) of the IRF1 promoter in Th1 cells resulting in up-regulation of IRF1. On the other hand, IRF1 regulates the expression of PD-L1 (programmed death ligand 1), an immune checkpoint, and it has been linked to the development of resistance to immunotherapy and chemotherapy. IRF1 is a promising therapeutic target in several disorders, and further studies will open new treatment avenues.
Description: IRF1 Luciferase Reporter HEK293 Cell Line is a HEK293 cell line that contains a firefly luciferase reporter under the control of three copies of the interferon gamma-activated sites (GAS) of the IRF1 (interferon regulatory factor 1) promoter stably integrated into HEK293 cell line. It is designed to monitor the activity of interferon gamma-induced signal transduction pathways in an HEK293 cell line. This cell line has been shown to be responsive to IFNγ and IFNα.
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Each vial contains ≥1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Target: IFN
Uniprot: P01579
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Coccia E. M., et al., 1999 JBC 274 (10): 6696-6703.Perevalova A., et al., 2024 Int J Mol Sci 25(4):2153.
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