MEF Starter Nucleofector® Kit

Mouse Embryonic Fibroblast Starter Nucleofector Kit

The Nucleofector™ 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. Due to significant diversity between different MEF clones, we have developed two Nucleofector™ Kits (1 and 2) and an easy-to-use Nucleofector™ Starter Kit. The Mouse Embryonic Fibroblast Nucleofector™ Starter Kit is used to determine which of the two Nucleofector™ Kits is best suited for transfecting a specific primary mouse embyonic fibroblast (MEF) clone in Nucleofector™ 2b Device.

Content:
•0.45 mL MEF 1 Nucleofector™ Solution
•0.45 mL MEF 2 Nucleofector™ Solution
•2 x 0.1 mL Supplement 1
•30 µg pmaxGFP™ Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
•10 Aluminum cuvette (100 µL)
•10 Single use pipettes

• SKU: LONVPD-1006
• Manufacturer: Lonza
• Manufacturer SKU: VPD-1006
• Package Unit: 10 reactions
• Quantity Unit: PAK
• Type: Nucleofection Kit