Description: This MAb recognizes the 56.5 kDa (CK10); 50 kDa (CK14); 50 kDa (CK15); 48 kDa (CK16); 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: AE-1
Immunogen: Human epidermal keratin
Antibody Reactivity: Cytokeratin, LMW
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
Ann N Y Acad Sci (1986) 468: 302-15. (Flow)
Entrez Gene ID: 374454
Expected AB Applications: Flow (intracellular) (published for clone)
Z-Antibody Applications: Flow, intracellular (published)/IHC, FFPE (verified)/WB (verified)
Verified AB Applications: IHC (FFPE) (verified)/WB (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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