Description: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse)
Conjugate: Purified, BSA-free
Concentration: 1 mg/mL
Storage buffer: PBS, no BSA, no azide
Clone: AHE-1
Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome
Antibody Reactivity: Eosinophil Peroxidase
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Leukocyte Biol (1989) 46:109-118. (RIA)
- J Exp Med (1991) 173, 117-126. (IHC, FFPE)
- Clin Exp Immunol (1993) 92:58-64. (ELISA, IP, functional studies, Flow)
- Gastroenterology (2011) 142(1): 15-20. (IHC, frozen)
Entrez Gene ID: 8288
Expected AB Applications: IHC (frozen)/ELISA (published for clone)/Flow, surface (published for clone)/IHC (FFPE) (published for clone)
Z-Antibody Applications: IF (verified)
Verified AB Applications: IF (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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