Description: Mouse Anti-Human Erythrocytes Clone SFL23.6 MAb is directed against an erythroid cell surface antigen. It shows a well-defined reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. Although the exact identity of the antigen has yet to be determined, it has been shown to be distinct from Glycophorin A. This MAb is useful in the diagnosis of erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: SFL23.6
Immunogen: Hepatocytes from a 20-22 week-old human fetus
Antibody Reactivity: Erythrocytes
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
Blood (1985) 66(3), 522-526. (Flow, IF)
Entrez Gene ID: Not Known
Expected AB Applications: Flow, surface (published for clone)/IF (published for clone)
Z-Antibody Applications: Flow, surface (published)/IF (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Flow cytometry: 0.5-1 ug/million cells/Immunofluorescence: 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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