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Application: Use in several in vitro assays focused on immunological research, drug development, and cancer studies, including cytotoxicity assays, immunotherapy screening, drug screening, and immune activation assays.
Background: T lymphocytes are composed of two subpopulations: αβ T-cells and γδ T-cells. They are distinguished by the expression of either an αβ TCR or a γδ TCR, respectively. αβ T-cells are the predominant subset of T cells in peripheral blood and recognize antigens presented by MHC (major histocompatibility complex) molecules. γδ T cells are less abundant and recognize antigens independently of MHC presentation. While both αβ T cells and γδ T cells contribute to cell cytotoxicity through distinct mechanisms to target and eliminate infected or abnormal cells, γδ T cells have a lower risk of causing GvHD (Graft-versus-Host Disease) when injected into humans and have demonstrated cytotoxicity against a wide range of tumor types. γδ TCRs are cell type-specific, with Vγ9Vδ2 being the predominant γδ T cell type in human peripheral blood. Vγ9Vδ2 T cells are involved mostly in immune responses to pathogens and long-term modulation of inflammation, and can recognize non-peptide phospho-antigens, alkylamines and synthetic amino-bisphosphonates. Vγ9Vδ2 T cells are being studied for the treatment of solid tumors and hematological disorders and are becoming a highly promising cancer therapy. Further studies on how best to utilize Vγ9Vδ2 T cells, and methods to enhance their presence, will open new therapeutic avenues for cancer and infections.
Description: Expanded Human Peripheral Blood Gamma Delta T Cells (Vγ9Vδ2), Frozen are Vγ9Vδ2 T cells enriched and expanded from human PBMCs (Peripheral Blood Mononuclear Cells) using the Vγ9Vδ2 T Cell Expansion Kit (BPS Bioscience #82551), and cryopreserved. Expanded Human Peripheral Blood Gamma Delta T Cells (Vγ9Vδ2), Frozen are >90 % pure Vγ9Vδ2 T cells (CD3+ and TCR Vγ9+cells), as measured by flow cytometry analysis. They can be used in cytotoxicity assays and other in vitro assays, after thaw in T Cell Medium (#78753).
Storage Stability: Expanded Human Peripheral Blood Gamma Delta T Cells (Vγ9Vδ2) are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.
Supplied As: Vial contains 2 x 106 cells or 10 x 106 cells in 1 ml of CryoStor® CS10 (Stemcell Technologies #100-1061)
Target: T Cell
Biosafety Level: BSL-1
References: Allison T. and Garboczi D., 2002 Molecular Immunology 38 (14): 1051-1061.Sawaisorn P., et al., 2024 Scientific Reports 14: 1291.
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