Description: Fibronectin is a dimeric glycoprotein of 440 kDa, which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This MAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product Origin: Animal - Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Conjugate: Purified, with BSA
Concentration: 0.2 mg/mL
Storage buffer: PBS, 0.05% BSA, 0.05% azide
Clone: HFN7.1
Immunogen: Human fibronectin purified from serum by affinity chromatography on gelatin-sepharose
Antibody Reactivity: Fibronectin
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- J Biol Chem (1991) 266(14): 23323-23328. (inhibition of integrin binding; epitope mapping)
- Anat Rec (1996) 245:068-676. (IF, frozen tissue)
- PNAS USA (2005) 102(17): 5953-5957. (functional studies)
- Biomaterials (2005) 26: 4523-4531. (IF; functional studies)
- Hybridoma (2006) 25(4): 202-208. (ELISA; WB)
Entrez Gene ID: 2335
Expected AB Applications: ELISA (published for clone)/Functional studies (published for clone)/IF (published for clone)/WB (published for clone)
Z-Antibody Applications: Functional studies (published)/IF (published)/ELISA (published)/IHC, FFPE (verified)/WB (published)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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