Products from BPS Bioscience require a minimum order value above 400€
Applications: Monitor glucocorticoid signaling pathway activity. Screen activators or inhibitors of the glucocorticoid signaling pathway.
Background: The glucocorticoid signaling pathway plays an important role in development, fluid homeostasis,cognition, immune response and metabolism. Glucocorticoids are a class of steroid hormonesthat bind to the glucocorticoid receptor, causing it to translocate to the nucleus. Upontranslocation, the receptor can regulate the transcription of a large number of genes, includingthose that regulate glucose metabolism and inflammatory responses.
Description: The Glucocorticoid Receptor Pathway Reporter Kit is designed for monitoring the activity of the glucocorticoid signaling pathway in cultured cells. The kit contains a transfection-ready expression vector for the glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR). This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low crossreactivity for other nuclear receptor pathways. The GAL4/UAS reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.
The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway-specific effects and background luciferase activity.
Storage Stability: >1 year from date of receipt when stored at -20°C
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Paguio A, et al. (2010) Improved Dual-Luciferase Reporter Assays for Nuclear Receptors. CurrChem Genomics. 4: 43-49.
English
Deutsch
