GRP94 Antibody

GRP94 Antibody
SKU
ROC200-501-F39
Packaging Unit
200 µg
Manufacturer
Rockland

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Application Note: Anti-Grp94 Antibody is tested for WB, IF, IP, and Flow cytometry. Expect a band approximately 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. This monoclonal antibody reacts with purified Grp94. Specific conditions for reactivity should be optimized by the end user.

Clone ID: 9G10

Concentration Value: 1mg/mL

Flow Cytometry Dilution: User Optimized

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 0.5µg/ml

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Immunogen: Grp94 Antibody was produced in rat by repeated immunizations with purified Grp94 isolated from chicken oviducts.

Physical State: Liquid (sterile filtered)

Purity and Specificity: Anti-GRP94 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with GRP94 from Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea pig, Hamster, Horse, Monkey, Pig, Rabbit, Sheep, Xenopus sources based on 100% homology with the immunizing sequence. Does not detect human Hsp90, Grp74, or GrpE from E.coli. Cross-reactivity with GRP94 from other sources has not been determined. Heat Shock research.

Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90. Both Hsp90 and Grp94 are calcium binding proteins. Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity. Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER

Low Endotoxin: No
More Information
SKU ROC200-501-F39
Manufacturer Rockland
Manufacturer SKU 200-501-F39
Package Unit 200 µg
Quantity Unit STK
Reactivity Human, Mouse (Murine), Rat (Rattus), Pig (Porcine), Sheep (Ovine), Monkey (Primate), Rabbit, Dog (Canine), Guinea Pig, Cow (Bovine), Hamster, Chicken, Frog (Xenopus Laevis), Horse (Equine)
Clonality Monoclonal
Application Immunofluorescence, Immunoprecipitation, Western Blotting, Flow Cytometry
Isotype IgG2a
Human Gene ID 374163
Host Rat
Conjugate Unconjugated
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