HLA-A*02:01 A549 Cell Line

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Application: /Use as a control for studying differences between HLA-A alleles. /Study T cell activation and responses to peptides presented by HLA-A*02:01.

Background: Human Leukocyte Antigen-A (HLA-A) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-A and β2-microglobulin (B2M). There are over 200 genes encoding HLA variants and this variability plays a critical role in adaptive immunity. HLA-A*02 is one of the most common members of class I types, with more than 300 variants HLA class I involved in presenting peptides that are typically between 8 to 11 amino acids. HLAA*02:01 can bind 15-mer peptides, which can then be recognized by T cells. Studies in SCLC (small cell lung cancer) patients have shown that an ATAD2 (ATPase family AAA domain-containing protein 2) immunopeptide can be used in HLA-A*02-01-restricted patients with high reactivity. HLA-A*02:01 abilities can be exploited in cancer therapy.

Description: The HLA-A*02:01 A549 Cell Line is an A549 human lung cancer cell line generated by selecting a single clone of B2M Knockout A549 cells (#82871) transduced with lentivirus expressing B2M (beta-2 microglobulin) HLA (human leukocyte antigen)-A*02:01 driven by an EF1a promoter (#82424). This cell line has been validated by flow cytometry and a co-culture assay response to Tebentafusp. Purchase of this cell line is for research purposes only; commercial use requires a separate license.

Host Cell Line: A549 is a human lung alveolar basal carcinoma cell line. Adherent epithelial cells.

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Target: HLA

Uniprot: P04439

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS82468
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82468
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human