HLA-A*03:01 A549 Cell Line

Products from BPS Bioscience require a minimum order value above 400€

Application: /Use as a control for studying differences between HLA-A alleles. /Study T cell activation and responses to peptides presented by HLA-A*03:01.

Background: Human Leukocyte Antigen-A (HLA-A) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-A and β2-microglobulin (B2M). There are over 200 genes encoding HLA variants and this variability plays a critical role in adaptive immunity. HLA-A*03 has been used as predictive biomarker in relation to the response of patients to ICI (immune checkpoint inhibitors). HLA-A*03:01 in particular has been linked to the development of stronger side effects to one of the COVID-19 vaccines. The understanding of the association between HLA alleles and diseases will allow a refinement of therapeutic strategies.

Description: The HLA-A*03:01 A549 Cell Line is a A549 human lung cancer cell line that was generated by selecting a single clone of B2M Knockout A549 cells (#82871) transduced with lentivirus expressing B2M (beta-2 microglobulin) HLA (human leukocyte antigen)-A*03:01 driven by an EF1a promoter (#82425). This cell line has been validated by flow cytometry. Purchase of this cell line is for research purposes only; commercial use requires a separate license.

Host Cell Line: A549 is a human lung alveolar basal carcinoma cell line. Adherent epithelial cells.

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Target: HLA

Uniprot: P04439

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS82469
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82469
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human