HLA-A*24:02 A549 Cell Line

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Application: Use as a control for studying the differences between HLA-A alleles. Study T cell activation and responses to peptides presented by HLA-A*24:02.

Background: Human Leukocyte Antigen-A (HLA-A) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-A and β2-microglobulin (B2M). There are over 200 genes encoding HLA variants and this variability plays a critical role in adaptive immunity. HLA-A*24:02 was found to be a common risk factor in antiepileptic drug cutaneous adverse effects, which resulted in a recommendation to perform HLA pre-screening prior to drug administration. Since its prevalence in Asia is higher than HLA-A*02:01, the use of an HLA-A*24:02- restricted peptide targeting AFP (alpha-fetoprotein) may prove beneficial for HCC (hepatocellular) treatment. The understanding of the association between HLA alleles and diseases will allow a refinement of therapeutic strategies.

Description: The HLA-A*24:02 A549 Cell Line an A549 human lung cancer cell line generated by selecting a single clone of B2M Knockout A549 cells (#82871) transduced with a lentivirus expressing B2M (beta-2 microglobulin) HLA (human leukocyte antigen)-A*24:02 driven by an EF1a promoter (#82427). This cell line has been validated by flow cytometry.

Host Cell Line: A549 is a human lung alveolar basal carcinoma cell line. Adherent epithelial cells.

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (#79796)

Target: HLA

Uniprot: P04439

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS82471
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82471
• Package Unit: 2 Vials
• Quantity Unit: PAK
• Host: Human