HLA-B*40:01 A549 Cell Line

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Application: Use as a control for studying differences between HLA-B alleles. Study T cell activation and responses to peptides presented by HLA-B*40:01.

Background: Human Leukocyte Antigen-B (HLA-B) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-B and β2-microglobulin (B2M). There are over 200 genes encoding HLA variants and this variability plays a critical role in adaptive immunity. HLA-B has been linked to autoimmune disorders and drug reactions. HLA-B*40:01 has been linked to ankylosing Spondylitis (AS), a chronic inflammatory rheumatic disorder that targets mainly the axial skeleton and sacroiliac joints. It has also been identified as crucial for the onset and development of anti-tumor responses. The understanding of the association between HLA alleles and diseases will allow a refinement of therapeutic strategies.

Description: The HLA-B*40:01 A549 Cell Line is an A549 human lung cancer cell line generated by selecting a single clone of B2M Knockout A549 cells (#82871) transduced with a lentivirus expressing B2M (beta-2 microglobulin) HLA (human leukocyte antigen)-B*40:01 driven by an EF1a promoter (#82432). This cell line has been validated by flow cytometry.

Host Cell Line: A549 is a human lung alveolar basal carcinoma cell line. Adherent epithelial cells

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage.

Supplied As: Each vial contains ˃1 x 106 cells in 1 ml of Cell Freezing Medium (#79796)

Target: HLA

Uniprot: P01889

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

• SKU: BPS82476
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82476
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human