Description: This MAb reacts with the HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. It binds a conformational epitope on HLA-DR, which depends on the correct folding of the α/β heterodimer. This MAb has been reported to block mixed lymphocyte reactions. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kD alpha (heavy) chain and a 28kD beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: L243
Immunogen: Activated human peripheral blood mononuclear cells
Antibody Reactivity: HLA-DRB
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Nature (1983) 304: 358-361. (IP, Flow)
- J Exp Med (2000) 191 (8): 1395-1412. (functional studies)
- J Clin Pathol (1991) 44:102-106. (IHC, frozen)
- Int J Cancer (2001) 93: 556-565. (ELISA, Flow, functional studies)
Entrez Gene ID: 3123
Expected AB Applications: ELISA (published for clone)/Flow, surface (published for clone)/Functional studies (published for clone)/IHC (frozen) (published for clone)/IP (published for clone)
Z-Antibody Applications: Flow, surface (published)/Functional studies (published)/IHC, frozen (published)/ELISA (published)/IP (published)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Optimal dilution for a specific application should be determined by user
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