Usage: This ELISA kit applies to the in vitro quantitative determination of Human GDH/GLDH concentrations in Serum, plasma and other biological fluids.
Tested Sample Types: Serum, plasma and other biological fluids
Type: Sandwich-ELISA
Assay Time: 3 h 30 min
Detection Type: Chemiluminescence
Sensitivity: 0.09 mIU/mL
Detection Range: 0.16-10 mIU/mL
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDH/GLDH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GDH/GLDH. You can calculate the concentration of Human GDH/GLDH in the samples by comparing the RLU value of the samples to the standard curve.
Cross reactivity: This kit recognizes Human GDH;GLDH in samples. No significant cross-reactivity or interference between Human GDH;GLDH and analogues was observed
Storage: 2-8℃/-20℃,6 months
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