Target Synonym: IL4;BCGF-1;BCGF1;BSF-1;BSF1
Usage: This ELISPOT kit is designed for the detection of the frequency of human IL-4secreting cells.
Tested Sample Types: Suspension cell
Assay Time: 3 h 30 min
Test Principle: This assay kit uses the Sandwich-ELISA principle. The PVDF membrane at the bottom of the micro ELISPOT plate has been re-coated with anti-human IL-4 antibodies. During the experiment, cell suspension and stimulants are added first, and IL-4 secreted by the cells during the incubation process will bind to the coated antibodies. Then wash away the cells, add biotinylated anti-human IL-4 antibodies and alkaline phosphatase conjugated avidin sequentially. The biotinylated anti-human IL-4 antibodies will bind specifically to the IL-4 which has already bounded, then avidin will bind specifically to biotin, together forming the complex. After free components are washed away, add chromogenic substrate (BCIP/NBT), BCIP undergoes hydrolysis under the catalysis of alkaline phosphatase and reacts with NBT, forming insoluble spots with color dark blue to blue-purple. Professional ELISPOT plate reader software can be used to analyze the result, calculating the frequency of human IL-4 secreting cells.
Cross reactivity: This kit recognizes Human IL-4 in samples.No significant cross-reactivity or interference between Human IL-4 and analogues was observed.
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