Description: This MAb is an excellent marker for all nuclei in cells and tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: NM106
Immunogen: Nuclei of HL60 cells
Antibody Reactivity: Human Nuclear Antigen
Entrez Gene ID: Not Known
Expected AB Applications: Flow (intracellular)
Z-Antibody Applications: Flow, intracellular/IF (verified)/IHC, FFPE (verified)
Verified AB Applications: IF (verified)/IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 0.5-1 ug/mL/Does not react with pig; others not known/Immunohistology formalin-paraffin 0.25-0.5 ug/mL/Immunocytochemistry acetone-fixed cells 0.25-0.5 ug/mL/Flow Cytometry 0.5-1 ug/million cells/0.1 mL
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