Human OPN (Osteopontin) ELISA Kit

Target Synonym: SPP1;BNSP;BSP1;ETA-1;Bone Sialoprotein I;Secreted Phosphoprotein 1;Early T-Lymphocyte Activation 1;

Usage: This ELISA kit applies to the in vitro quantitative determination of Human OPN concentrations in serum, plasma and other biological fluids.

Tested Sample Types: serum, plasma and other biological fluids

Type: Sandwich-ELISA

Assay Time: 3 h 30 min

Detection Type: Colormetric

Sensitivity: 0.19 ng/mL

Detection Range: 0.31-20 ng/mL

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OPN. You can calculate the concentration of Human OPN in the samples by comparing the OD of the samples to the standard curve.

Cross reactivity: This kit recognizes Human OPN in samples. No significant cross-reactivity or interference between Human OPN and analogues was observed

Storage: 2-8℃/-20℃,12 months

Citation Link 1: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8826575/

Citation Link 2: https://www.sciencedirect.com/science/article/pii/S1525730420302199

Citation Link 3: https://www.sciencedirect.com/science/article/pii/S1896112622000426

• SKU: ELSE-EL-H1347-48
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-EL-H1347-48
• Package Unit: 48 T
• Quantity Unit: PAK
• Application: ELISA