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Application: / Depletion of TCRα/β+ T cells from PBMCs while other immune cell subsets, such as γδ T cells, B cells, NK cells, and monocytes remain in the cell suspension./ Isolation of TCR αβ+ T cells from PBMCs for downstream applications where the presence of magnetic beads is not a concern.
Background: TCR α/β T cells are a major subset of T lymphocytes that express the αβ T-cell receptor (TCR) and play a central role in adaptive immunity by recognizing peptide antigens presented by major histocompatibility complex (MHC) molecules. These cells include CD4⁺ helper T cells and CD8⁺ cytotoxic T cells, which coordinate immune responses and target infected or malignant cells, respectively. In peripheral blood mononuclear cells (PBMCs), TCR αβ+ T cells typically constitute around 60–80% of total lymphocytes, with variations depending on individual immune status and conditions.
Description: The Human TCRα/β T Cell Depletion Kit is designed to magnetically deplete human TCRα/β+ T cells from a complex immune cell population. This kit is optimized for the positive selection of TCRα/β T cells from normal human peripheral blood mononuclear cells (PBMCs). First, TCRα/β+ cells are labeled using Anti-TCRα/β Antibody-Biotin followed by binding to Streptavidin Magnetic Beads. The labeled cell suspension is then placed in on a Cell Isolation Magnetic Tube Rack. The magnetically isolated TCRα/β+ cells are retained on the side of the tube, while the unlabeled cells remain in suspension. As a result, this cell fraction is effectively depleted of TCRα/β+ cells.
Format: #82941-1 (1x10e8 cells)Catalog #NameAmountStorage Cell Isolation Magnetic Beads 500 µl2-8°C Anti-human TCR α/β Antibody 500 µl2-8°C785635x Cell Isolation Buffer 25 ml2-8°C#82941-2 (1x10e9 cells)Catalog #NameAmountStorage Cell Isolation Magnetic Beads 5 ml2-8°C Anti-human TCR α/β Antibody 5 ml2-8°C785635x Cell Isolation Buffer 250 ml2-8°C
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Target: TCR
Uniprot: Q6ISU1
Warnings: This product is for research purposes only and not for human or therapeutic use. This product should be considered hazardous and is harmful by inhalation, in contact with skin, eyes, clothing, and if swallowed. If contact occurs, wash thoroughly.
Biosafety Level: Not applicable (BSL-1)
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