Human VEGF-A ELISA Kit

Shelf Life: 6 months from date of receipt

Uniprot ID: P15692

Background: VEGFA is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Assay Range: 8.23 pg/ml - 6000 pg/ml

Assay Type: Sandwich ELISA

Detection Method: Biotinylated-Streptavidin-HRP

Sample Type: serum_plasma_cell_culture_supernatant

Contents:

• Human VEGF-A Pre-Coated 96-well Strip Plate, 1 each
• Human VEGF-A Standard vial, 2 each
• Human VEGF-A Detection Antibody
• Assay Diluent(s)
• Streptavidin-HRP Solution, 200µl
• TMB Substrate, 12 ml
• Stop Solution, 12 ml
• 20X Wash Buffer, 25 ml
• Sealing Tape, 2 sheets

• SKU: BEYE82-010
• Manufacturer: Bethyl Laboratories
• Manufacturer SKU: E82-010
• Package Unit: 96 wells
• Quantity Unit: STK
• Reactivity: Human
• Application: ELISA
• Human Gene ID: 7422