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Encompassing Amino Acids: 109-270
Application: Useful for binding assays.
Assay Conditions: Assay was performed according to IL1RL1:IL33 [Biotinylated] Inhibitor Screening Chemiluminescence Assay Kit (#82856) with increasing amounts of biotinylated IL-33.
Background: IL-33 is a cytokine of the IL-1 family that is released in response to external triggers such as trauma, allergen exposure, and infections. IL-33 exists in a reduced or oxidized state, which trigger different signaling pathways. When in its reduced state (IL-33red) it binds to IL-1RL1 ((interleukin 1 receptor like 1), also named IL-33R or ST2 (receptor serum-stimulated 2). IL-1RL1 exists as both a membrane bound protein or a truncated soluble form. Binding of IL-33 to IL-1RL1 initiates a signaling pathway that involves NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) and MAPK (mitogen-activated protein kinase), and results in release of pro-inflammatory cytokines and chemokines. The soluble form of IL-1RL1 can act as a decoy receptor for IL-33. Dysregulation in the levels of IL-33 is implicated in the pathology of inflammatory and infectious diseases, including COVID-19, COPD (chronic obstructive pulmonary disease), and asthma. The involvement of IL-33 in these diseases has made it a high value therapeutic target, with strategies such as antibodies being developed to inhibit the binding of IL-33 to its receptor. One example is tozorakimab (MEDI3506), currently being evaluated as treatment for COPD. Further studies on IL-33 and its receptor will likely result in new therapies for IL-33 related disorders.
Description: Recombinant human IL-33 (Interleukin 33), encompassing amino acids 109-270. This construct contains an N-terminal His-tag (6xHis) followed by an Avi-Tag™. This protein is enzymatically biotinylated using Avi-Tag™ technology. The recombinant protein was affinity purified.
Endotoxin Level: 121 EU/mg
Format: Aqueous buffer solution.
Formulation: 8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol.
Genbank: NM_033439.4
Purity: ≥75%
Storage Stability: >6 months at –80°C.
Tags: His-Avi
Target: IL-33
Uniprot: O95760
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
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