Clone: OX-42
Specificity: Recognizes the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain. Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes(1). XXX recommend the use of MCA275EL for use in functional studies.
Antibody Modification: FITC
Presentation: PBS, 0.09% Sodium Azide, 1% BSA
Immunogen: Rat resident peritoneal macrophages.
Gene: ITGAM / CD11b
Description: CD11b antibody LS-C45060 is an FITC-conjugated mouse monoclonal antibody to rat CD11b (ITGAM). Validated for Flow.
Usage: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Synonyms: ITGAM, Antigen CD11b (p170), CD11B, CD11b antigen, CR3A, Integrin alpha-M, MAC-1, MO1A, Neutrophil adherence receptor, MAC1A, SLEB6, CR-3 alpha chain
Recommended Storage: Store at 4°C or at -20°C. Store undiluted. Do not use frost-free freezers. Protect from light. Microcentrifugation recommended if solution contains precipitate.
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