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Encompassing Amino Acids: 866-1154 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted at 30°C for 1 hour in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Tyr6, Invitrogen) using Z-Lyte assay conditions.
Description: Human Jak1 (Janus kinase 1), also known as JTK3, GenBank Accession No. NM_002227, a.a. 866-1154(end) with N-terminal GST-Tag, MW= 59 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.
Genbank: NM_002227
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P23458
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rodig, S.J., et al. (1998). Cell 93 (3): 373-8.
2. Haan, C., et al. (2001). J. Biol. Chem. 276 (40): 37451-8.
3. Miyazaki, T., et al. (1994). Science 266 (5187): 1045-7
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