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Encompassing Amino Acids: 532-1132
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.5, 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 2 µM substrate (Tyr Peptide 6, Z-lyte kinase assay kit from Invitrogen), and 0-100 ng enzyme at 37°C for 1 hour.
Description: Human Jak2 (GenBank Accession No. NM_004972), containing the catalytic and the pseudo kinase domain (a.a. 532-1132) with N-terminal His-GST-tag, MW=98 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.4 mM KCl, 0.04% Tween-20, 3 mM DTT, 10% glycerol and 250 mM Imidazole
Genbank: NM_004972
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST-tags
Uniprot: O60674
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005).
2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).
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