Target: KDEL
Conjugate: Biotin
Product Type: Monoclonal
Clone Number: 2D6
Immunogen: KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
Purification: Protein G Purified
Storage Buffer: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
Concentration: 1 mg/ml
Specificity: Detects KDEL tagged proteins.
Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). Grp78 and Grp94 and PDI all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER.
References: 1. Ozawa K., et al. (2008) Mol Pharmacol. 74:1610.2. Austin R.C., et al. (2003) J Biol Chem. 278: 17438.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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