Transfection

Transfection

The Principle of Nucleofector™ Technology:

By applying electrical pulses, small pores are generated short-term in the cell membrane. An interplay of preprogrammed Nucleofection protocols and coordinated reagents result in perfectly adapted conditions for a successful transfection for the particular cell type used. The substrate is not only taken up by the cytoplasm, but also by the nucleus due to the optimized conditions. This process allows high transfection rates with an efficiency of up to 99%.

We offer the following platforms:

  • For maximum flexibility: 4D Nucleofector (X, Y and LV units)
  • For medium-throughput: add-on 96-well shuttle
  • For high throughput: HT Nucleofector
  • The base unit: Nucleofector 2b

The Nucleofector kits contain special transfection cuvettes, Nucleofector reagents (buffer and supplement) and the control plasmid pmaxGFP. The buffer and its supplement are optimized specifically for respective primary cells.

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  1. 4D-Nucleofector™ LV Reservoir Rack
    4D-Nucleofector LV Reservoir Rack
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    Sku LONAAK-3001
    Manufact. SkuAAK-3001
    Packaging Unit1 Stück
  2. 4D-Nucleofector™ 96-well Unit
    The medium-throughput module for the 4D-Nucleofector® Platform suited for transfection of hard-to-transfect cell lines or primary cells in 96-well format
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    Sku LONAAF-1003S
    Manufact. SkuAAF-1003S
    Packaging Unit1 Stück
  3. 4D-Nucleofector® LogWare Gen 2
    4D-Nucleofector® LogWare Gen 2
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    Sku LONSAAF-1002
    Manufact. SkuSAAF-1002
    Packaging Unit1 Stück
  4. Electroporation cuvettes, 4 mm gap, sterile & individually packaged, 50 pcs
    Electroporation cuvettes, 4 mm gap, sterile & individually packaged, 50 pcs
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    Sku LUB12358-347
    Manufact. Sku12358-347
    Packaging Unit50 pieces
  5. Electroporation cuvettes, 2 mm gap, sterile & individually packaged, 50 pcs
    Electroporation cuvettes, 2 mm gap, sterile & individually packaged, 50 pcs
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    Sku LUB12358-346
    Manufact. Sku12358-346
    Packaging Unit50 pieces
  6. Electroporation cuvettes, 1 mm gap, sterile & individually packaged, 50 pcs
    Electroporation cuvettes, 1 mm gap, sterile & individually packaged, 50 pcs
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    Sku LUB12358-345
    Manufact. Sku12358-345
    Packaging Unit50 pieces
  7. 4D-Nucleofector™ Core Unit
    4D-Nucleofector™ Core Unit
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    Sku LONAAF-1003B
    Manufact. SkuAAF-1003B
    Packaging Unit1 Stück
  8. 4D-Nucleofector™ X Unit
    4D-Nucleofector™ X Unit
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    Sku LONAAF-1003X
    Manufact. SkuAAF-1003X
    Packaging Unit1 Stück
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