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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: LYN A is a member of the Src-family of protein kinases that participate in signaling pathways of a variety of cell surface receptors and are localized to the cytoplasmic side of the plasma membrane (1). LYN A is biosynthetically transported to the plasma membrane via the Golgi pool of caveolin along the secretory pathway. The trafficking of LYN A from the Golgi apparatus to the plasma membrane is inhibited by deletion of the kinase domain but not by kinase inactivation. The kinase domain of LYN A plays a role in LYN A trafficking besides catalysis of substrate phosphorylation. cDNA microarray analysis revealed that LYN A may be involved in the changes that are related to tumor pathogenesis (2).
Description: Recombinant human LYN A, full length, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: NM_002350
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P07948
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kasahara, K. et al: Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain. J. Cell Biol., 2004;165(5):641-52.
2. Baran, C.P. et al: The inositol 5'-phosphatase SHIP-1 and the Src kinase Lyn negatively regulate macrophage colony stimulating factor-induced Akt activity. J. Biol. Chem. 2003; 278(40):38628-36
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