Description: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin: Animal - Mus musculus (mouse), Bos taurus (bovine)
Conjugate: CF740
Concentration: 0.1 mg/mL
Storage buffer: PBS, 0.1% rBSA, 0.05% azide
Clone: M2-7C10
Immunogen: Recombinant hMART-1 protein
Antibody Reactivity: MART-1/Melan-A
References: Note: References for this clone sold by other suppliers may be listed for expected applications.
- Int J Cancer (1998) 75, 517-524. (IHC, frozen; IHC, cytospin)
- Cancer Cytopathol (1999) 87(1): 37-42. (IHC, FFPE; IHC, cytospin)
- Mol Med Rep (2011) 4: 799-803. (WB; IF; IHC)
- World J Immunol (2013) 3(3): 62-67. (Flow, intracellular)
Entrez Gene ID: 2315
Expected AB Applications: Immunofluroescence (published for clone)/Flow (intracellular) (published for clone)/IHC (frozen) (published for clone)/WB (published for clone)
Z-Antibody Applications: Flow, intracellular (published)/IF (published)/IHC, frozen (published)/IHC, FFPE (verified)/WB (published)
Verified AB Applications: IHC (FFPE) (verified)
Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunofluorescence: 1-2 ug/mL/Does not react with mouse or rat, others not tested/Immunohistology formalin-fixed 0.5-1 ug/mL/Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes/Flow Cytometry 0.5-1 ug/million cells/0.1 mL/Western blotting 0.5-1 ug/mL/Optimal dilution for a specific application should be determined by user
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