Target Synonym: METTL;C12orf;C12orf1;METTL1;D1075 like gene product;FLJ95748;Methyltransferase like 1;Methyltransferase like protein 1;Methyltransferase-like protein 1;TRM8;TRMB_HUMAN;TRMT8;tRNA (guanine N(7) ) methyltransferase;tRNA (guanine(46)-N(7))-methyltransferase;tRNA (guanine-N(7)-)-methyltransferase;tRNA(m7G46) methyltransferase;tRNA(m7G46)-methyltransferase;YDL201w
Background: tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.
Immunogen: Recombinant Human METTL1 protein
Buffer: 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0
Dilution: WB 1:500-1:2000;IP 4-6 μL/mg of lysate
Calculated MW: 34 kDa
Observed MW: 34 kDa
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