Products from BPS Bioscience require a minimum order value above 400€Applications: Screen for activators or inhibitors of the Notch signaling pathway.
Study effects of RNAi or gene overexpression on the activity of the Notch pathway.
Background: The Notch signaling pathway controls cell fate decisions in vertebrate and invertebratetissues. Notch signaling is triggered through the binding of a transmembrane ligand,such as Delta like ligand 4 (DLL4) or jagged 1 (JAG1) to a Notch transmembranereceptor (Notch1, Notch2, Notch3, Notch4) on a neighboring cell. This results inproteolytic cleavage of the Notch receptor, releasing the constitutively active intracellulardomain of Notch (NICD). NICD translocates to the nucleus and associates withtranscription factors CSL (CBF1, RBPJ, Suppressor of Hairless, Lag-1) and thecoactivator, Mastermind, to turn on transcription of Notch responsive genes.
Concentration: The Reporter and Negative Control Reporter are provided at 60 ng DNA/µl (500 µl each). The Notch1 Expression Vector and Negative Control Expression Vector are provided at 100 ng DNA/µl (250 µl each).
Description: The Notch Pathway Reporter kit is designed for monitoring the activity of Notch signaling pathway in the cultured cells. The kit contains the transfection-ready expression vector for Notch1 with the entire extracellular domain deleted (Notch1DE). Inside the cells, the Notch1DE can be cleaved by -secretase and active Notch1 NICD is released to nucleus. The kit also contains a CSL (CBF1/RBP-J) luciferase reporter vector, which is a Notch pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized CSL responsive element upstream of a minimal promoter. The CSL (CBF1/RBP-J) reporter is premixed with constitutively expressing Renilla (sea pansy) luciferase vector, which serves as an internal positive control for transfection efficiency.The kit also includes a non-inducible firefly luciferase vector premixed with constitutivelyexpressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical for determining pathway specific effects and background luciferase activity.
Storage Stability: Stable at least 12 months from date of receipt, when stored as directed (-20°C)
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lu FM et al. (1996). Proc. Natl. Acad. Sci. USA 93(11): 5663-5667.
2. Kanungo et al (2008). J.Neurochem. 106 2236.Application Reference:
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