MyoD1 (Rhabdomyosarcoma Marker) (rMYD712), CF740 conjugate, 0.1mg/mL

MyoD1 (Rhabdomyosarcoma Marker) (rMYD712), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC742329-100
Packaging Unit
100 uL
Manufacturer
Biotium

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Description: This antibody recognizes a phosphoprotein of 45 kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Mus musculus (mouse), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: rMYD712

Immunogen: Recombinant full-length human MyoD1 protein

Antibody Reactivity: MyoD1

Entrez Gene ID: 4654

Z-Antibody Applications: IHC, FFPE (verified)

Verified AB Applications: IHC (FFPE) (verified)

Antibody Application Notes: Only nuclear staining should be considered as evidence of skeletal muscle differentiation. Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC742329-100
Manufacturer Biotium
Manufacturer SKU BNC742329-100
Package Unit 100 uL
Quantity Unit STK
Reactivity Human
Clonality Recombinant
Application Immunohistochemistry
Isotype IgG1 kappa
Host Mouse
Conjugate Conjugated, CF740
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