Target: NCC
Conjugate: ATTO 390
Product Type: Polyclonal
Immunogen: Produced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
Swiss-Prot: P55018, P55017
Purification: Affinity Purified
Storage Buffer: 640.91mM DMSO, 136.36mM Ethanolamine, and 9.09mM Sodium Bicarbonate in 90.9% PBS
Concentration: 1 mg/ml
Specificity: Detects ~160kDa.
Cellular Localization: Membrane
Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
References: 1. Vallon V., Schroth J, Lang F, Kuhl D and Uchida S. (2009) Am J Physiol Renal Physiol. 297(3): F704-712.2. Tiwari S., et al. (2009) Am J Nephrol. 30(6): 554-562.3. Chee K.N., Vorontsova I., Lim J.C., Kistler J. and Donaldson P.J. (2010) Mol Vis. 16:800-812.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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