Application Note: Anti-Neurofilament L antibody is tested for use in Western Blotting, ICC and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 68 kDa in size corresponding to the Neurofilament L proteins in Western Blot in the appropriate cell lysate or extract.
Clone ID: DA2
Concentration Value: Titrated value sufficient to run approximately 10 mini blots.
IF Microscopy Dilution: 1:100
Western Blot Dilution: 1:1000
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Immunogen: Anti-NF-L Monoclonal Antibody was produced by repeated immunizations with preparation of swine intermediate filaments.
Physical State: Liquid
Purity and Specificity: Anti-NF-L antibody is directed against NF-L protein. The antibody is protein G purified from concentrated clarified cell culture supernatant. The antibody has been directly tested for reactivity in bovine, chicken, human, mouse and rat tissues. Cross reactivity with NF-L from other species has not been determined.
Background: NF-L antibody detects Neurofilament L. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease. Anti-Neurofilament L antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.
Low Endotoxin: No
Other: User Optimized
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