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Applications: Monitor intracellular calcium levels. Screen for activators or inhibitors of PKC/ Ca2+ pathway. Screen for agonists or antagonists of T cell receptors. Control for immune checkpoint NFAT reporter cell lines.
Background: The nuclear factor of activator T cells (NFAT) family of transcription factors plays an importantrole in immune response. T cell activation through the T cell synapse results in calcium influx.Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin,which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the aminotermini of NFAT proteins. This results in a conformational change that exposes a nuclearlocalization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperatewith other proteins to bind to DNA.
Description: The NFAT Reporter - Jurkat Cell Line contains a firefly luciferase gene under the control of theNFAT response element stably integrated into Jurkat cells. This cell line has been validated forresponse to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful asa control cell line for other NFAT reporter cell lines expressing various immune checkpointreceptors.
Host Cell Line: Jurkat
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Supplied As: Each vial contains ~2x106 cells in 1 ml of 10% DMSO.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-1
References: 1. Clipstone NA, Crabtree GR. Nature. 1992 Jun 25;357(6380):695-7.
2. Lyakh, L., et al. Mol Cell Biol. 1997 May;17(5):2475-84.
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