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Encompassing Amino Acids: 425-719
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK5 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm
Background: PAK5 is a protein kinase of the STE20 family that is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. Associated with microtubule networks and induces microtubule stabilization. Its subcellular localization is tightly regulated during cell cycle progression.
Description: Human PAK5 (GenBank Accession No. NM_177990), a.a. 425-719, MW=33.9 kDa, expressed in E. coli.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 7.0, 50 mM NaCl, 10% glycerol, 5 mM DTT
Genbank: NM_177990
Purity: ≥95%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q9P286
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Li,Y., et al., PLoS ONE 3 (7), E2707 (2008).
2. Ewing,R.M., et al., Mol. Syst. Biol. 3, 89 (2007).
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