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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted usingBPS's PARP1 Assay Kit (Catalog #80551) at room temperature for 1 hour.
Description: Human PARP1 (GenBank Accession No. NM_001618), full length with N-terminal GST-tag, MW = 140 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 20% glycerol, and 3 mM DTT.
Genbank: NM_001618
Purity: ≥80%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P09874
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Aguilar-Quesada R, et al., 2007. Curr Med Chem. 14(11):1179-87.
2. Pacher P. and Szabó C. 2007. Cardiovasc Drug Rev. 25(3):235-60.Application Reference(s):De Vos, M., et al. J Biol Chem. 2014 Jun 6;289(23):16223-38. doi: 10.1074/jbc.M113.527424. Poly(ADP-ribose) polymerase 1 (PARP1) associates with E3 ubiquitin-protein ligase UHRF1 and modulates UHRF1 biological functions (2014)Larsson, E.A., et al. J Med Chem. 2013 Jun 13;56(11):4497-508. doi: 10.1021/jm400211f. Fragment-based ligand design of novel potent inhibitors of tankyrases (2013)
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