Target Synonym: CD49 antigen-like family member D;CD49d;ITGA4;Integrin alpha-4;Integrin alpha-IV;VLA-4 subunit alpha
Usage: Each lot of this antibody is quality control tested by flow cytometric analysis.
The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Citation Link 1:
https://789.bio/ec/y9KteHCitation Link 2:
Human peripheral blood lymphocytes are stained with PE/Elab Fluor® 594 Anti-Human CD49d Antibody[9F10] (filled gray histogram) or PE/Elab Fluor® 594 Mouse IgG1, κ Isotype Control (empty black histogram).Background: CD49d is a 150 kD α integrin chain known as α4 integrin or VLA-4 α chain. It forms a heterodimer with either integrin β1 (α4β1, VLA-4) or β7 (α4β7). CD49d is expressed broadly on T lymphocytes, B lymphocytes, monocytes, thymocytes, eosinophils, basophils, mast cells, NK cells, dendritic cells, and some non-hematopoietic cells, but not on normal red blood cells, platelets or neutrophils. VLA-4 binds to VCAM-1 (CD106) and fibronectin. α4β7 is the receptor for VCAM-1 and MAdCAM-1. CD49d participates in mononuclear cell trafficking to endothelial sites of inflammation and has roles in cell-cell interactions and cell adhesion to extracellular matrices. CD49d is involved in lymphocyte migration, T cell activation, and hematopoietic stem cell differentiation. CD49d is a marker to isolate pure populations of Treg cells due to its absence on Foxp3+ cells.
Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
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