PE/Elab Fluor® 594 Anti-Mouse F4/80 Antibody(CI:A3-1)

Target Synonym: Gpf480;Adgre1;Adhesion G protein-coupled receptor E1;Cell surface glycoprotein F4/80;EGF-like module receptor 1;Emr1

Usage: Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Storage: This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.

Citation Link 1: https://789.bio/ec/aT0ib9

Citation Link 2: C57BL/6 murine abdominal macrophages elicited by starch broth are stained with APC Anti-Mouse/Human CD11b Antibody and PE/Elab Fluor^® 594 Anti-Mouse F4/80 Antibody (Left). Macrophages are stained with APC Anti-Mouse/Human CD11b Antibody and PE/Elab Fluor^® 594 Rat IgG2b, κ Isotype Control (Right).

Background: F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.

• SKU: ELSE-AB-F0995P-100
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: E-AB-F0995P-100
• Package Unit: 100 Tests
• Quantity Unit: PAK
• Reactivity: Mouse (Murine)
• Clonality: Monoclonal
• Application: Flow Cytometry
• Isotype: Rat IgG2b κ
• Human Gene ID: 13733
• Host: Rat
• Conjugate: Conjugated, PE (Phycoerythrin), Elab Fluor594