Clone: TLD-3A12
Specificity: Recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes. Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This clone has been reported as suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2 M boric acid, pH7.0. Has been shown to be cross-reactive with endothelial cells derived from rhesus macaque.
Antibody Modification: FITC
Presentation: PBS, 0.09% Sodium Azide, 1% BSA
Immunogen: Activated, Lewis rat derived microglial cells
Gene: PECAM-1 / CD31
Description: CD31 antibody LS-C43480 is an FITC-conjugated mouse monoclonal antibody to rat CD31 (PECAM-1). Validated for Flow and IHC. Cited in 1 publication.
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
Synonyms: PECAM1, CD31, CD31 antigen, EndoCAM, PECA1, PECAM-1, GPIIA
Recommended Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Protect from light. Microcentrifugation recommended if solution contains precipitate.
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