Formulation: Each vial, when reconstituted to 500 μl with water, contains 50 μg of pertussis toxin in in 0.01 M sodium phosphate, 0.05 M sodium chloride, pH 7.0
Purity: ≥90% estimated by SDS-PAGE
Formula Weight: 0
Shelf life (days): 730
CAS Number: 70323-44-3
Notes: Pertussis toxin (islet-activating protein) is a toxin, first isolated from B. pertussis, that is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the α subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/o proteins.{31502,31505} Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals and induce EAE, an animal model of multiple sclerosis.{31501,31504} Pertussis toxin was first described as an islet-activating protein because it caused a sustained potentiation of the secretory response of pancreatic islet cells to various stimuli that stimulate Gi-linked α-adrenergic receptors.{31500,31503}
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