Immunogen: Synthetic peptide from the C-terminus of rat VAChT conjugated to BSA
Concentration: n/a
Storage buffer: Lyophilized; reconstitute in 100 µl dist. water
Additional info: The antiserum against the vesicular acetylcholine transporter is a unique immunohistochemical marker for cholinergic nerves, more specific than the commonly used acetylcholine esterase (AchE), since it does not react with postsynaptic neurons, and is more sensitive than choline acetyltransferase (ChAT). The antiserum recognizes VAChT both in the CNS and PNS of rat and mouse.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes staining.Positive control: frozen sections of rat small intestine.
Applications: IHC-fr, IHC-P (1:800-1:1,200)