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Protein Tag: N-His
Uniprot: Q99JW5
Accession: Q99JW5-1
Background: Epithelial cell adhesion molecule (EpCAM,CD326) is a type I transmembrane glycoprotein that functions as a homophilic,epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion,EpCAM is also involved in cellular signaling,cell migration,proliferation,and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas,and has become a therapeutic target.
Bio Acitivity: Not validated for activity
Sequence: Glu24-Thr266
Purity: > 90% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 27.7 kDa
Observed MW: 30 kDa
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