Products from BPS Bioscience require a minimum order value above 400€Applications: Generate stable cell line expressing human PSMA with puromycin selection
Background: PSMA (prostate-specific membrane antigen, also known as Folate hydrolase 1, FOLH1), is highly expressed in prostate cancer cells and is used as a diagnostic and prognostic indicator of prostate cancer. The enzyme has folate hydrolase and peptidase activity. It plays a role in prostate cancer progression through the PI3K-Akt and MAPK-ERK1/2 pathways. PSMA/FOLH1is used in the clinic as a target for PET (positron emission tomography) imaging of prostate cancer whereas radioactive analogs are used for the treatment of prostate cancer. It is also expressed in other tumor types and in a few normal tissues. PSMA/FOLH1 is the target of CAR-T cells and bi-specific antibodies currently under development.
Description: The PSMA Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. The particles contain a human PSMA (NM_ 004476.3) driven by a CMV promoter and a puromycin selection marker (Figure 1).
Figure 1: Schematic of the lenti-vector used to generate the PSMA Lentivirus.
Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥10
7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Uniprot: Q04609
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
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