Application Note: WB: 1:1000-1:5000. ELISA: 1:50000-1:100000. IHC: 1:500-1:2500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Specificity/Sensitivity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
Form: Liquid
Buffer (with preservative): 20mM Potassium Phosphate, 150mM NaCl, 1% BSA, 0.01% Gentamicin Sulfate. Do NOT add Sodium Azide!.
Concentration: 2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species: Human
Immunogen: Native Human IgG whole molecule
Target Isotype: IgG
Purification: Purified by antigen-affinity chromatography followed by solid phase adsorption(s) to remove any unwanted reactivities
Conjugation: HRP
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