Usage: This ELISA kit applies to the in vitro quantitative determination of Rat, Chicken CORT concentrations in serum, plasma, urine.
Tested Sample Types: serum, plasma, urine
Type: Competitive-ELISA
Assay Time: 2 h 30 min
Detection Type: Colormetric
Sensitivity: 0.24 ng/mL
Detection Range: 0.39-25 ng/mL
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat, Chicken CORT. During the reaction, Rat, Chicken CORT in the sample or standard competes with a fixed amount of Rat, Chicken CORT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat, Chicken CORT. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat, Chicken CORT in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Cross reactivity: This kit recognizes Rat, Chicken CORT in samples.No significant cross-reactivity or interference between Rat, Chicken CORT and analogues was observed
Storage: 2-8℃/-20℃,12 months
Citation Link 1:
https://www.sciencedirect.com/science/article/pii/S0269749122020589Citation Link 2:
https://www.sciencedirect.com/science/article/pii/S221112472200211XCitation Link 3:
https://www.sciencedirect.com/science/article/pii/S0048969721017915
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