Rb1 (Tumor Suppressor Protein) (RB1/2313R), CF740 conjugate, 0.1mg/mL

Rb1 (Tumor Suppressor Protein) (RB1/2313R), CF740 conjugate, 0.1mg/mL
SKU
BTMBNC742313-500
Packaging Unit
500 uL
Manufacturer
Biotium

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Description: This antibody recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Product Origin: Animal - Oryctolagus cuniculus (domestic rabbit), Bos taurus (bovine)

Conjugate: CF740

Concentration: 0.1 mg/mL

Storage buffer: PBS, 0.1% rBSA, 0.05% azide

Clone: RB1/2313R

Immunogen: Recombinant full-length human Rb1 protein

Antibody Reactivity: Rb1

Entrez Gene ID: 5925

Z-Antibody Applications: IHC, FFPE (verified)

Verified AB Applications: IHC (FFPE) (verified)

Antibody Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody/Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT/Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes/Optimal dilution for a specific application should be determined by user
More Information
SKU BTMBNC742313-500
Manufacturer Biotium
Manufacturer SKU BNC742313-500
Package Unit 500 uL
Quantity Unit STK
Reactivity Human, Mouse (Murine)
Clonality Recombinant
Application Immunohistochemistry
Isotype IgG
Host Rabbit
Conjugate Conjugated, CF740
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