Research Areas: Cell Biology
Uniprot: Q99174
Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Form: Liquid or Lyophilized powder
Tag Info: C-terminal 6xHis-tagged
Purity: Greater than 90% as determined by SDS-PAGE.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Molecular Weight: 23.7 kDa
Gene Names: CUTA
Organism: Fusarium solani subsp. cucurbitae (Neocosmosporum cucurbitae)
Source: Yeast
Expression Region: 17-230aa
Protein Length: Full Length of Mature Protein
Target Protein Sequence: LPTSNPAQELEARQLGRTTRDDLINGNSASCADVIFIYARGSTETGNLGTLGPSIASNLESAFGTDGVWIQGVGGAYRATLGDNALPRGTSSAAIREMLGLFQQANTKCPDATLIAGGYSQGAALAAASIEDLDSAIRDKIAGTVLFGYTKNLQNRGRIPNYPADRTKVFCNVGDLVCTGSLIVAAPHLAYGPDARGPAPEFLIEKVRAVRGSA
Endotoxin: Not test.
Relevance: Catalyzes the hydrolysis of cutin, a polyester that forms the structure of plant cuticle. Allows pathogenic fungi to penetrate through the cuticular barrier into the host plant during the initial stage of the fungal infection.
Reference: "Effect of disruption of a cutinase gene (cutA) on virulence and tissue specificity of Fusarium solani f. sp. cucurbitae race 2 toward Cucurbita maxima and C. moschata."Crowhurst R.N., Binnie S.J., Bowen J.K., Hawthorne B.T., Plummer K.M., Rees-George J., Rikkerink E.H., Templeton M.D.Mol. Plant Microbe Interact. 10:355-368(1997)
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