Recombinant Human ACTN4 protein(His tag)

Protein Tag: N-His & C-His

Uniprot: O43707

Accession: O43707-1

Background: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions.May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.

Bio Acitivity: Not validated for activity

Sequence: Met1-Lys300

Purity: > 90% as determined by reducing SDS-PAGE.

Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.

Calculated MW: 34.0 kDa

Observed MW: 38 kDa

• SKU: ELSPDEH100765-500
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: PDEH100765-500
• Package Unit: 500μg
• Quantity Unit: STK
• Reactivity: Human
• Host: Escherichia Coli