Recombinant Human BAX Protein(Trx Tag)

Protein Tag: N-Trx

Uniprot: Q07812

Accession: Q07812

Background: BCL2 associated X (BAX) is traditionally thoμght to be regulated by anti-apoptotic BCL-2 family members. BCL-2-associated X protein (BAX) is a critical apoptotic regulator that can be transformed from a cytosolic monomer into a lethal mitochondrial oligomer, The pro-apoptotic BCL-2 protein BAX commits human cells to apoptosis by permeabilizing the outer mitochondrial membrane. BAX activation has been sμggested to require the separation of helix alpha5 from alpha6 - the 'latch' from the 'core' domain - among other conformational changes. BCL-2-associated X (BAX) protein acts as a gatekeeper in regulating mitochondria-dependent apoptosis. Under cellular stress, BAX becomes activated and transforms into a lethal oligomer that causes mitochondrial outer membrane permeabilization (MOMP).

Bio Acitivity: Not validated for activity

Sequence: Met1-Trp158

Purity: > 95% as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Endotoxin: < 10 EU/mg of the protein as determined by the LAL method

Calculated MW: 37.4 kDa

Observed MW: 35 kDa

• SKU: ELSPDEH100644-1
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: PDEH100644-1
• Package Unit: 1mg
• Quantity Unit: STK
• Reactivity: Human
• Host: Escherichia Coli