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Protein Tag: C-His
Uniprot: P49327
Accession: P49327
Background: FASN (Fatty Acid Synthase, also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a beta-ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta-ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207-2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.
Bio Acitivity: Not validated for activity
Sequence: Ala 2201-Gly 2511
Purity: > 95% as determined by reducing SDS-PAGE.
Formulation: Lyophilized from sterile PBS, pH 7.4.
Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Endotoxin: < 10 EU/mg of the protein as determined by the LAL method.
Calculated MW: 34.1 kDa
Observed MW: 35 kDa
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